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. 2018 Dec 12;54(3):821–832. doi: 10.3892/ijo.2018.4666

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Copyright: © Kasioumi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Comparison of cell migration of used cell lines with wound healing technique. (A) HeLa and HeLa-siRNA-Hsp70, A549, A549-siRNA-Hsp70, MCF7 and MCF-7-siRNA-Hsp70 were cultured in 6 cm petri dishes. After scratching the cells, the wound healing was recorded 12, 24 and 36 h later. Cellular migration was calculated as described in the Materials and methods. Each point represents the mean value ± SD of 3 measurements in 3 separate experiments. In all points, except that of 0 h, the P-values were <0.01. (B) Photographic example of wound healing using HeLa and HeLa-siRNA-Hsp70 cells at the same time periods.