Table I.
Primer sequences of MDM2, p53, p21, Bad, Bax, Cyt-c, caspase-3, Cox-2, MMP-2, MMP-9 and GAPDH.
| Genes | Primer sequence (5′-3′) |
|---|---|
| MDM2 | F: GAAAGATGGAGCAAG |
| R: GAGGTGGTTACAGCA | |
| p53 | F: GTCATCTTCTGTCCCTTCCC |
| R: ACCTCAGGCGGC TCATAG | |
| p21 | F: GCG ATG GAA CTT CGACTT TGT |
| R: GGG CTT CCT CTT GGA GAAGAT | |
| Bad | F: CCCAGAGTTTGAGCCGAGTG |
| R: CCCATCCCTTCGTCGTCCT | |
| Bax | F: GCTTCAGGGTTTCATCCAG |
| R: GGCGGCAATCATCCTCTG | |
| Bcl-2 | F: TACTTAAAAAATACAACATCACAG |
| R: GGAACACTTGATTCTGGTG | |
| Cyt-c | F: GATCACCCCCAGCCTCCCTTATC |
| R: AAAATAGAGAATTTAAAAGGCCTAAC | |
| Caspase-3 | F: CTGGACTGTGGCATTGAGAC |
| R: ACAAAGCGACTGGATGAACC | |
| Cox-2 | F: TTCCAGATCCAGAGCTCATTAAA |
| R: CCGGAGCGG GAAGAACT | |
| MMP-2 | F: CCGCAGTGACGGAAAGATGT |
| R: GCCACGAGGAACAGGCTGTA | |
| MMP-9 | F: ACGCACGACGTCTTCCAGTAC |
| R: ACCTGGTTCAACTCACTCCGG | |
| GAPDH | F: CAACTACATGGTTTACATGTTC |
| R: GCCAGTGGACTCCACGAC |
MDM2, murine double minute 2; Bcl-2, B-cell lymphoma-2; Bax, BCL2-associated X; Cyt-c, cytochrome c; Cox-2, cyclooxygenase 2; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse.