Figure 5.

miR-3664-5P inhibits tumor growth in vivo. (A) BALB/c nude mice (age, 6 weeks) were subcutaneously transplanted with LV-miR-3664-5P MGC803 cells (1×10⁷) in the left and LV-NC MGC803 cells (1×10⁷) in the right groin (n=10). (B) Tumor volume was determined every 5 days following transplantation and mice were sacrificed following 30 days. Tumor volume was calculated using: (width² × length)/2. (C) Tumor weight was measured following sacrifice (D) A tail vein xenograft model was constructed to investigate metastasis. (E) Representative images of hematoxylin and eosin staining of tumor tissues from mice subcutaneously injected with miR-3664-5P overexpression cells or NC cells (magnification, x200). (F) The number of metastatic lesions was calculated in each group. Data are presented as the mean ± standard deviation (n=10). ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. LV-NC. NC, negative control; LV, lentivirus; LV-NC, cells transfected with NC LV; LV-miR-3664-5P, cells transfected with miR-3664-5P overexpression LV; miR, microRNA; GC, gastric cancer.