CDX2 expression is correlated with FXR and MUC2 expression in gastric IM tissues. (A) Immunohistochemical staining of CDX2, MUC2 and FXR in gastric mucosal tissues with or without IM. Scale bar, 100 µm. (B) CDX2, (D) MUC2 and (F) FXR staining was classified into negative and positive, and the percentage of tissues in each group was shown. IRS of (C) CDX2, (E) MUC2 and (G) FXR staining in normal mucosa and IM tissues. Reverse transcription-quantitative polymerase chain reaction was performed to determine the mRNA expression levels of (H) CDX2, (I) MUC2 and (J) FXR in mucosal tissues with or without IM. Correlations between the mRNA expression levels of CDX2 and (K) MUC2 (r=0.3602; P<0.05) and (L) FXR (r=0.3674; P<0.05) in gastric IM tissues. Data are presented as the means ± standard deviation from three independent experiments. **P<0.01. CDX2, caudal-related homeobox transcription factor 2; FXR, farnesoid X receptor; IM, intestinal metaplasia; IRS, immunoreactivity score; MUC2, mucin 2.