Table II.
Gene name | Sequence (5′–3′) |
---|---|
Hsa-miR-134 | |
RT primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGTTGGTGTC |
Forward | ACACTCCAGCTGGGCACCAA |
Hsa-miR-218 | |
RT primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGUGUAGAAA |
Forward | ACACTCCAGCTGGGTTGTGCTTGATCTAA |
Hsa-miR-488 | |
RT primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGTTGAGAGT |
Forward | ACACTCCAGCTGGGCCCAGATAATGGCA |
Hsa-miR-159 | |
RT primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGTCCTACCC |
Forward | ACACTCCAGCTGGGCAGACTTGGCCAT |
U6 | |
Forward | CTCGCTTCGGCAGCACA |
Reverse | AACGCTTCACGAATTTGCGT |
E-cadherin | |
Forward | AAAGGCCCATTTCCTAAAAACCT |
Reverse | TGCGTTCTCTATCCAGAGGCT |
Vimentin | |
Forward | AGTCCACTGAGTACCGGAGAC |
Reverse | CATTTCACGCATCTGGCGTTC |
α-SMA | |
Forward | CATTTCACGCATCTGGCGTTC |
Reverse | CCATCAGGCAGTTCGTAG |
GAPDH | |
Forward | TGCACCACCAACTGCTTAGC |
Reverse | GGCATGGACTGTGGTCATGAG |
α-SMA, α-smooth muscle actin; miR-134, microRNA-134; RT, reverse transcription.