Skip to main content
. 2018 Dec 12;54(3):916–928. doi: 10.3892/ijo.2018.4665

Table II.

Forward and reverse primers used for RT-quantitative polymerase chain reaction.

Gene name Sequence (5′–3′)
Hsa-miR-134
 RT primer GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGTTGGTGTC
  Forward ACACTCCAGCTGGGCACCAA
Hsa-miR-218
  RT primer GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGUGUAGAAA
  Forward ACACTCCAGCTGGGTTGTGCTTGATCTAA
Hsa-miR-488
  RT primer GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGTTGAGAGT
  Forward ACACTCCAGCTGGGCCCAGATAATGGCA
Hsa-miR-159
  RT primer GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGTCCTACCC
  Forward ACACTCCAGCTGGGCAGACTTGGCCAT
U6
  Forward CTCGCTTCGGCAGCACA
  Reverse AACGCTTCACGAATTTGCGT
E-cadherin
  Forward AAAGGCCCATTTCCTAAAAACCT
  Reverse TGCGTTCTCTATCCAGAGGCT
Vimentin
  Forward AGTCCACTGAGTACCGGAGAC
  Reverse CATTTCACGCATCTGGCGTTC
α-SMA
  Forward CATTTCACGCATCTGGCGTTC
  Reverse CCATCAGGCAGTTCGTAG
GAPDH
  Forward TGCACCACCAACTGCTTAGC
  Reverse GGCATGGACTGTGGTCATGAG

α-SMA, α-smooth muscle actin; miR-134, microRNA-134; RT, reverse transcription.