Figure 6.

MTV ventilation increased non-canonical Wnt signaling protein expression in the lungs of A/J sensitive mice. (A) p-RhoA, p-JNK and (B) ROCK1 protein expression was measured by western blotting. β-actin was the loading control (n=4-6). ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. control. (C) Immunohistochemical staining for p-JNK in the lungs following mechanically ventilating with LTV and MTV for 6 h in A/J mice. LTV, low tidal volume; MTV, moderate tidal volume; p-, phosphorylated; JNK, c-Jun N terminal kinase; ROCK1, Rho-associated protein kinase 1; RhoA, Ras homolog gene family, member A.