Figure 1.

25-HC has no effects on GC cell proliferation and apoptosis in vitro. (A) AGS cells or MGC-803 cells were treated with the indicated concentrations of 25-HC for 24, 48 or 72 h followed by CCK-8 assay. The absorbance at 450 nm was measured. (B) AGS cells or MGC-803 cells were treated with 25-HC at the indicated concentrations for 48 h and apoptosis was examined by FITC-Annexin V/PI staining. The fluorescence intensity of FITC-Annexin V was plotted on the x-axis, and PI was plotted on the y-axis. The statistical analysis of apoptotic GC cells (FITC⁺) were calculated. (C) Cells were treated with 25-HC for 48 h and total cellular proteins were extracted for western blot analysis to detect the Bcl-2 and Bax expression levels. Results were obtained from 3 independent experiments and are expressed as the means ± SEM. 25-HC, 25-hydroxycholesterol; GC, gastric cancer.