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. 2019 Jan 11;54(3):966–980. doi: 10.3892/ijo.2019.4684

Figure 4.

Figure 4

25-HC arrests the cell cycle of GC cells at S-phase following 5-FU treatment. AGS cells or MGC-803 cells were treated with 25-HC with or without 5-FU (5 µM) for 48 h. Cell cycle distribution was examined by using PI staining and flow cytometry analysis. Representative flow cytometric analysis of cell cycle distribution and the statistical analysis is shown. Results were obtained from 3 independent experiments and are expressed as the means ± SEM and statistical significance was determined by one-way ANOVA followed by Dunnett's test. Representative images are presented. *P<0.05, **P<0.01 and ***P<0.001. 25-HC, 25-hydroxycholesterol; GC, gastric cancer.