Figure 5.

25-HC promotes GC cell migration and invasion in vitro. (A) Invasion assays of AGS cells or MGC-803 cells treated with various concentrations of 25-HC were performed using cell culture chambers with 8-µm pore filter. After 36 h, the cells invaded to the underside of the membrane were quantitated by cell counting in 5 pre-determined fields. (B) The migratory ability of the AGS cells was determined by wound healing assay. Monolayers of AGS cells were wounded by a 200-µl plastic tip and then incubated for a further 24 h in 5% FBS medium with different concentrations of 25-HC. (C) AGS cells were treated with the indicated concentrations of 25-HC for 24 h or were treated with 5 µM 25-HC for the indicated times before total cellular proteins were extracted for western blot analysis to detect the MMPs expressions. Results were obtained from 3 independent experiments and are expressed as the means ± SEM and statistical significance was determined by one-way ANOVA followed by Dunnett's test. Representative images are presented. ^*P<0.05 and ^***P<0.001. 25-HC, 25-hydroxycholesterol; GC, gastric cancer.