Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Experimental model | Zebrafish: Tg(gsc::EGFP-CAAX) |
Smutny et al., 2017 | NA | |
| Experimental model | Zebrafish: Tg(gsc::Turbo-RFP) |
Sako et al. (2016) | ZDB-ALT- 161005–1 |
|
| Experimental model | Zebrafish: MZfz7a/b |
Quesada-Hernández et al. (2010) | ZDB-FISH- 150901–19586 |
|
| Experimental model | Zebrafish: MZfz7a/b;Tg (gsc::EGFP-CAAX) |
this paper | NA | |
| Experimental model | Zebrafish: MZfz7a/b;Tg (gsc::Turbo-RFP) |
this paper | NA | |
| Oligonucleotides |
casanova
morpholino GCATCCGGTCGAGATACATGCTGTT |
Gene Tools | ZDB-MRPHLNO -050818–2 |
|
| Recombinant DNA reagent |
pcDNA 3.1-Opto-Fz7 plasmid for mRNA synthesis |
this paper | NA | |
| Recombinant DNA reagent |
pcDNA 3.1-Opto-Fz7-mCherry plasmid for mRNA synthesis |
This paper | NA | |
| Recombinant DNA reagent |
pCS2-Ndr2 plasmid for mRNA synthesis |
Rebagliati et al., 1998 | NM_139133.1 | |
| Recombinant DNA reagent |
pCS2-Wnt11-3xmCherry | this paper | NM_001144804.1 | |
| Recombinant DNA reagent |
pCS2-Fz7a-mNeongreen | this paper | NM_131139.2 | |
| Recombinant DNA reagent |
pCS2-Pk1-venus | Carreira-Barbosa et al., 2003 | NM_183342.2 | |
| Recombinant DNA reagent |
pCS2-Dvl2-eGFP | other | NM_212648.1 | courtesy of Masazumi Tada |
| Antibody | ß-catenin clone 15B6 | Sigma | C7207, RRID:AB_476865 |
|
| Antibody | Alexa Fluor 568 Goat Anti-Mouse IgG (H + L) Antibody |
Molecular Probes | A11004, RRID: AB_141371 |
|
| Antibody | GFP mAb3E6 | Molecular Probes | A11120, RRID:AB_221568 |
|
| Antibody | Alexa Fluor 488 Goat Anti-Mouse IgG (H + L) Antibody |
Molecular Probes | A11001 | |
| chemical compound |
9-cis-retinal | Sigma | R5754 | |
| Cell culture reagent |
DMEM/F12 | Sigma | DF-041-B |