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. 2019 Jan 21;43(3):1321–1330. doi: 10.3892/ijmm.2019.4071

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Copyright: © Ren et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of FGF-21 on autophagy makers in H9c2 cardiomyocytes. (A) Western blot analysis of LC3-II protein levels. (B) Densitometric analysis of LC3-II levels. (C) Western blot analysis of Beclin-1 protein levels. (D) Densitometric analysis of Beclin-1 levels. (E) Western blot analysis of p62 protein levels. (F) Densitometric analysis of p62 levels. ^*P<0.05 vs. control group, ^#P<0.05 vs. H/R group and ^&P<0.05 vs. H/R + FGF-21 group. Values are presented as mean ± standard deviation. Experiments were repeated in triplicate. FGF-21, fibroblast growth factor-21; H/R, hypoxia/reoxygenation; 3-MA, 3-methylad-enine; LC3-II, lipid modified microtubule-associated proteins 1A/1B light chain; p62, sequestosome 1.