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. 2019 Jan 21;43(3):1321–1330. doi: 10.3892/ijmm.2019.4071

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Copyright: © Ren et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of FGF-21 on autophagic flux in H9c2 cardiomyocytes. (A) H9c2 cardiomyocytes transfected with adenovirus harboring tandem fluorescent mCherry-GFP-LC3 (Ad-LC3-H9c2) for 24 h were subjected to different treatments. Representative images of immunofluorescent H9c2 cardiomyocytes expressing mRFP-GFP-LC3. Green fluorescence represents GFP and red fluorescence represents mRFP. (B) Semi-quantitative analysis of autophagosomes (yellow dots in merged images) and autolysosomes (red only dots in merged images). ^*P<0.05 vs. control group, ^#P<0.05 vs. H/R group and ^&P<0.05 vs. H/R + FGF-21 group. Values are expressed as mean ± standard deviation. Experiments were repeated in triplicate. FGF-21, fibroblast growth factor-21; H/R, hypoxia/reoxygenation; 3-MA, 3-methyladenine; GFP, green fluorescent protein; mRFP, monomeric red fluorescent protein.