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. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: Metallomics. 2012 Jun 13;4(7):669–678. doi: 10.1039/c2mt20025b

Fig. 4. Structural location and copper transport activity of the ATP7B mutants.

Fig. 4

(A) The A-domain model shown as blue cartoons. A874 is shown as pink atom-spheres, and surrounding residues in blue spheres. (B) The P-domain model in red, with L1083 shown as pink spheres. (C) The membrane domain in green, with R969 as pink spheres. (D) Development of black pigment by tyrosinase is indicative of copper transport by ATP7B. ATP7B-A874V shows no copper transport activity. ATP7B-R969Q shows activity/intensity of color similar to wtATP7B. ATP7B-L1083F shows variable color intensity as indicated by arrows. Cells transfected with tyrosinase plasmids will not develop color and serve as a negative control for the assay. (E). Quantitation of average pigment intensity using ImageJ.