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. 2019 Feb 6;5(2):eaau7130. doi: 10.1126/sciadv.aau7130

Fig. 4. c-Abl kinase activates CRL4-DDA1 to reduce the level of HBV polymerase.

Fig. 4

(A) Immunoblot for polymerase protein level in HEK293T cells overexpressing Flag-Abl and knocking down DDB1 or CUL4A/4B. (B) Immunoblot for polymerase protein level in HEK293T cells overexpressing Flag-Abl and knocking down Cdt2. (C) Ubiquitination of polymerase by IP in Huh7 cells expressing HA polymerase, Flag-Abl, Myc-ub, and siDDB1, siCUL4A, siCUL4B, or a combination of siCUL4A and siCUL4B and treated with MG132 for 8 hours. IP-Myc (top) or total cell lysates (bottom) were then analyzed for the indicated proteins. (D) Immunoblot for polymerase protein level in HEK293T cells overexpressing Flag-Abl and Flag-DDA1. (E) Immunoblot for polymerase protein level in HEK293T cells overexpressing Flag-Abl and knocking down DDA1. (F) Immunoblot for polymerase protein level in HEK293T cells overexpressing Flag-Abl, Myc-DDB1 wild type, and triple mutant (TM).