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. 2019 Feb 6;5(2):eaau7130. doi: 10.1126/sciadv.aau7130

Fig. 5. Inhibition of c-Abl kinase activates HBV replication by stabilizing the level of HBV polymerase.

Fig. 5

(A) Strategy (see text for description). (B) HepG2 cells or (C) Huh7 cells were cotransfected with indicated plasmids and were treated with or without imatinib for 24 hours before harvest, whole-cell lysates were prepared for Western blotting (bottom), and capsid-associated viral DNAs were quantitated by real-time PCR (top). Mean copy number from cells only transfected with compHBV was set to 100% and compared with others (n = 3 per group). (D) HepG2 cells or (E) Huh7 cells were cotransfected with indicated plasmids and were treated with DMSO, MG132, or MLN4924 for 8 hours before harvest; whole-cell lysates were prepared for Western blotting (bottom); and capsid-associated viral DNAs were quantitated by real-time PCR (top). Mean copy number from cells treated with DMSO was set to 100% and compared with others (n = 3 to 4 per group). (F) HepG2 cells or (G) Huh7 cells were transfected with indicated siRNAs and plasmids, whole-cell lysates were prepared for Western blotting (bottom), and capsid-associated viral DNAs were quantitated by real-time PCR (top). Mean copy number from cells transfected with control siRNA was set to 100% and compared with others (n = 3 to 4 per group). *P < 0.05, **P < 0.01, and ***P < 0.001.