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. Author manuscript; available in PMC: 2019 Nov 10.
Published in final edited form as: J Control Release. 2018 Sep 24;289:114–124. doi: 10.1016/j.jconrel.2018.09.020

Figure 6.

Figure 6.

(A) Survival curve and (B) body weight of mice challenged with 10,000 ffu of A/Puerto Rico/8/1934 H1N1 flu strain. Mice were vaccinated on Day 0 and Day 21 with phosphate-buffered saline (PBS), soluble M2e + MF59-like AddaVax (solM2e + squalene), M2e-loaded microparticles (M2e MPs (20, 40, or 60%)) + cGAMP-loaded microparticles (cGAMP MPs (60%)), or MPs containing both M2e and cGAMP (M2e/cGAMP MPs (60%)). All MPs were composed of acetalated dextran (Ace-DEX), and percentages indicate AceDEX MP relative cyclic acetal coverages. The M2e and cGAMP doses were 10 μg and 1 μg, respectively. Mice were challenged on Day 56, monitored for up to 14 days, and euthanized upon 20% loss of their starting weight. Statistical significance with respect to PBS is presented as **p < 0.01 and ***p < 0.001. Statistical significance with respect to M2e/cGAMP MPs (60%) is presented as †p < 0.05 and ††p < 0.01. Statistical significance with respect to solM2e + squalene is presented as ‡p < 0.05 and ‡‡‡p < 0.001 (n=14–15). Statistical analysis was performed using the Log-rank Mantel-Cox Test.