KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-Phospho-ACC S79 | Cell Signaling Technology | Cat# 3661 |
| Rabbit (40H9) anti-Phospho-AMPKα T172 | Cell Signaling Technology | Cat# 2535 |
| Rabbit polyclonal anti-Phospho-Raptor S792 | Cell Signaling Technology | Cat# 2083 |
| Rabbit polyclonal anti-ACC | Cell Signaling Technology | Cat# 3662 |
| Rabbit polyclonal anti-AMPKα | Cell Signaling Technology | Cat# 2532 |
| Rabbit polyclonal anti-AMPKα1 | Cell Signaling Technology | Cat# 2795 |
| Rabbit polyclonal anti-AMPKα2 | Cell Signaling Technology | Cat# 2757 |
| Rabbit (D60C5) anti-LKB1 | Cell Signaling Technology | Cat# 3047 |
| Rabbit (24C12) anti-Raptor | Cell Signaling Technology | Cat# 2280 |
| Rabbit polyclonal anti-Cleaved Caspase 3 | Cell Signaling Technology | Cat# 9661 |
| Rabbit (D8H5) anti-Ulk1 | Cell Signaling Technology | Cat# 8054 |
| Rabbit (D1H4) anti-Phospho-Ulk1 S555 | Cell Signaling Technology | Cat# 5869 |
| Rabbit polyclonal anti-Phospho-S6 S235/236 | Cell Signaling Technology | Cat# 2211 |
| Mouse (JY2) anti-TXNIP | MBL | Cat# K0205-3 |
| Rabbit anti-Tfe3 | Cell Signaling Technology | Cat# 14779 |
| Rabbit polyclonal anti-4EBP1 | Cell Signaling Technology | Cat# 9452 |
| Rabbit polyclonal anti-Phospho-S6K (T389) | Cell Signaling Technology | Cat# 9205 |
| Rabbit (5G10) anti-S6 | Cell Signaling Technology | Cat# 2217 |
| Mouse (7G6C5) anti-Hdac3 | Cell Signaling Technology | Cat# 3949 |
| Rabbit polyclonal anti-Tfeb | Bethyl Laboratories | Cat# A303-673A |
| Rabbit polyclonal anti-AMPKα1 | EMD Millipore | Cat# 07-350 |
| Rabbit polyclonal anti-AMPKα2 | EMD Millipore | Cat# 07-363 |
| Mouse (AC-15) anti-Actin | Sigma-Aldrich | Cat# A5441 |
| Mouse (B-5-1-2) anti-α-Tubulin | Sigma-Aldrich | Cat# T5168 |
| Rabbit (D7D11) anti-Phospho-ACC S79 | Cell Signaling Technology | Cat# 11818 |
| Rat (BU1/75 (ICR1)) anti-BrdU | Abcam | Cat# 6326 |
| Rabbit polyclonal anti-Glut1 | Alpha Diagnostics | Cat# GT11-A |
| Rabbit (D57.2.2E) anti-Phospho-S6 S235/236 | Cell Signaling Technology | Cat# 4858 |
| Rat (GL2A7) anti-Lamp2 | Abcam | Cat# 13524 |
| Rabbit polyclonal anti-Neu1 | GeneTex | Cat# GTX64524 |
| Rabbit polyclonal anti-Slc17a5 | GeneTex | Cat# GTX115822 |
| Rabbit (D3U4C) anti-LC3A/B | Cell Signaling Technology | Cat# 12741 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| 991 | Glixx Laboratories | Cat# GLXC-09267 |
| Phenformin hydrochloride | Sigma-Aldrich | Cat# P7045 |
| Aicar | Toronto Research Chemicals | Cat# A611700 |
| Rotenone | Sigma-Aldrich | Cat# R8875 |
| D-Luciferin potassium salt | PerkinElmer | Cat# 122796 |
| BrdU | Sigma | B5002 |
| Critical Commercial Assays | ||
| Cyquant | LifeTechnologies | Cat# C35011 |
| Annexin V-PE Apoptosis Detection | BD Pharmingen | Cat# 559763 |
| FITC BrdU Flow Kit | BD Pharmingen | Cat# 559619 |
| Deposited Data | ||
| Raw data files from RNA seq on 368T1 KP NSCLC cells | Mendeley (data.mendeley.com) | DOI 10.17632/gj763hyrny.1 |
| Raw data files from RNA seq on 634T KP NSCLC cells | Mendeley (data.mendeley.com) | DOI 10.17632/3cy28w7v9k.1 |
| TCGA Research Network | (Cancer Genome Atlas Research, 2014) | http://cancergenome.nih.gov/. |
| Experimental Models: Cell Lines | ||
| 368T1 mouse NSCLC cells | Dr. Monte Winslow | (Winslow et al., 2011) |
| 634T mouse NSCLC cells | Dr. Kwok Wong | (Liu et al., 2013) |
| 821T4 mouse NSCLC cells | Dr. Kwok Wong | (Liu et al., 2013) |
| 807LN mouse NSCLC cells | Dr. Kwok Wong | (Liu et al., 2013) |
| Experimental Models: Organisms/Strains | ||
| KrasLSLG12D/+ mice (K Luc) on FVB | (Shackelford et al., 2013) | N/A |
| KrasLSLG12D/+; p53fl/fl; R26LSLLuc/Luc mice (KP Luc) on FVB | (Shackelford et al., 2013) | N/A |
| KrasLSLG12D/+; LKB1fl/fl; R26LSLLuc/Luc mice (KL Luc) on FVB | (Shackelford et al., 2013) | N/A |
| KrasLSLG12D/+; p53fl/fl; LKB1fl/fl; R26LSLLuc/Luc mice (KPL Luc) on FVB | This paper | N/A |
| AMPKα1fl/fl mice | Dr. Benoit Violet | N/A |
| AMPKα2fl/fl mice | Dr. Benoit Violet | N/A |
| KrasLSLG12D/+; R26LSLLuc/Luc; AMPKα1fl/fl mice on FVB | This paper | N/A |
| KrasLSLG12D/+; R26LSLLuc/Luc; AMPKα2fl/fl mice on FVB | This paper | N/A |
| KrasLSLG12D/+; R26LSLLuc/Luc; AMPKα1fl/fl; AMPKα2fl/fl mice on FVB | This paper | N/A |
| KrasLSLG12D/+; p53fl/fl; R26LSLLuc/Luc; AMPKα1fl/fl; AMPKα2fl/fl mice on FVB | This paper | N/A |
| SCID/Beige mice: PrkdcscidLystbg-J/Crl:Fox Chase | Charles River | Cat# 250 |
| Oligonucleotides | ||
| LCM_PCR Rec_AMPKα1_Fwd: CCCATGAGCTCCAGAAGAAG | This paper | N/A |
|
LCM_PCR Rec_AMPKα1_Rev: GCCAGACACAGGTGAAGACA |
This paper | N/A |
|
LCM_PCR Rec_AMPKα2_Fwd: GATCTGTCTGCTTCTGCGTTC |
This paper | N/A |
|
LCM_PCR Rec_AMPKα2_Rev: CGATGGCCGCTCTAGATAAC |
This paper | N/A |
|
LCM_PCR Lox_AMPKα1_Fwd: AATAGCCCATGAGCTCCAGA |
This paper | N/A |
|
LCM_PCR Lox_AMPKα1_Rev: TGCAGCCCTACACTGAAATG |
This paper | N/A |
|
LCM_PCR Lox_AMPKα2_Fwd: ATGGCTTGGCGCTGTCTA |
This paper | N/A |
|
LCM_PCR Lox_AMPKα2_Rev: ACTTTCTGGCCAGGCAGATG |
This paper | N/A |
| See Table S1 for additional oligonucleotides. | ||
| Recombinant DNA | ||
| pSpCas9(BB)-2A-Puro (PX459) | Addgene | Cat# 48139 |
| Lenti-Pgk-Cre | Dr. Tyler Jacks | (DuPage et al., 2009) |
| human AMPKα1 cDNA in DONR vector | Human Orfeome collection | |
| Software and Algorithms | ||
| Inform v2.1 | Cambridge Research and Instrumentation | |
| STAR aligner v2.4.0k | (Dobin et al., 2013) | |
| HOMER | (Heinz et al., 2010) | |
| EdgeR package v3.6.8 | (Robinson et al., 2010) | |
| GSEA | (Mootha et al., 2003) | |
| Gene Cluster 3.0 | (deHoon et al., 2004) | |
| Java TreeView v1.1.6r4 | (Saldanha et al., 2004) | |
| DAVID tool | (Huang et al., 2009) | http://david.ncifcrf.gov |
| cBioPortal | (Cerami et al., 2012) (Gao et al., 2013) | www.cbioportal.org |
| Other | ||