Fig. 2.
Cell viability and produced nitric oxide concentration in RAW 264.7 murine macrophage cells treated with root extracts from HPG and SCG. (A) Cell viability of RAW 264.7 cells. (B) Concentration of nitric oxide in RAW 264.7 cells (μM). Cell viability was assessed by MTT assay with different sample concentrations (400 μg/mL, filled square; 200 μg/mL, open square; 100 μg/mL, open square). Cell viability (%) = {(absorbance of sample − absorbance of blank)/(absorbance of control − absorbance of blank)} × 100. Concentration of nitrite (μM) = (absorbance of sample at 540 nm + 0.0008)/0.0071, R2 = 0.9999. (−), Non-treated with LPS; (+), treated with LPS (0.1 μg/mL). Alphabet notation represents significant difference among samples based on Duncan’s multiple test (p < 0.05)