Figure 1.

Ex vivo neutrophil extracellular trap (NET) formation in antineutrophil cytoplasmic antibodies (ANCA)−associated vasculitis (AAV) and systemic lupus erythematosus (SLE). Paul-Karl-Horan (PKH)26-labelled neutrophils (red) derived from a healthy donor were exposed to 10% serum of patients with AAV or SLE for 4 hours to induce NET formation. Extracellular DNA was stained with SYTOXgreen (green) and NET formation was imaged with immunofluorescence confocal microscopy. Images of AAV- (a) and SLE-induced (b) NET formation are shown at original magnification ×10; bar = 20 um.