Skip to main content
. 2019 Jan 28;176(5):737–750. doi: 10.1111/bph.14560

Figure 6.

Figure 6

The effect of kazinol U on SP600125‐induced MITF and tyrosinase expression. (A) B16F10 cells were treated with kazinol U or DMSO for 30 min and cultured with SP600125 (20 μM) for 30 min. (B) After pretreatment with kazinol U or DMSO for 1 h, cells were treated with SP600125 for 7 h. (C) Cells pretreated with kazinol U or DMSO for 3 h were treated with SP600125 for 24 h. JNK phosphorylation and MITF and tyrosinase expression were detected by Western blot analysis (n ≥ 5). (D and E) Cells were pretreated with kazinol U (20 μM) or DMSO for 3 h before the addition of SP600125 (20 μM) for 48 h. Tyrosinase activity was determined by measuring dopachrome formation. Cell pigmentation was photographed using a Canon EOS 60D (A–D: n = 5). One‐way ANOVA with a post hoc Newman–Keuls test was used for the statistical analysis; *P < 0.05.