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. 2019 Jan 28;176(5):737–750. doi: 10.1111/bph.14560

Figure 8.

Figure 8

The effect of kazinol U on melanogenesis in zebrafish. (A) The experimental design for the zebrafish model. (B–D) Zebrafish embryos were treated with kazinol U (10–50 μM), phenylthiourea (PTU) (200 μM), arbutin (2 mM) or DMSO from 24 to 72 hpf. The pigmentation of the embryos was observed under a stereomicroscope. To determine the tyrosinase activity and melanin content, 50 zebrafish embryos were treated with each compound from 24 to 72 hpf and lysed with 1% Triton X‐100 in DPBS. After centrifugation, the supernatants were used to determine the tyrosinase activity. Pellets were dissolved in 500 μL of 1N NaOH/10% DMSO for 2 h at 80°C and used for measuring the absorbance at 405 nm using Victor3 (C: n = 6, D: n = 5). One‐way ANOVA with a post hoc Newman–Keuls test was used for the statistical analysis; *P < 0.05.