Figure 6.

Seq-915_x4024 Suppresses the Expression of Col I, Col III, p-Smad2, and p-Smad3 In Vivo

On days 3, 6, 9, and 12 after transplantation, the wound tissues were harvested for further detection. (A) Western blotting was used to estimate the protein expression of Col I and Col III. (B) Real-time qRT-PCR was used to estimate the mRNA expression of Col I. (C) Real-time qRT-PCR was used to estimate the mRNA expression of Col III. (D) Real-time qRT-PCR was used to estimate the mRNA expression of Smad2. (E) Real-time qRT-PCR was used to estimate the mRNA expression of Smad3. (F) Western blotting was used to estimate the protein expression of Smad2 and Smad3. (G) Western blotting was used to estimate the protein expression of p-Smad2 and p-Smad3. The results represent the means of the values. Bars indicate SD. *p < 0.05, statistical significance between groups.