Overall ability of urine amino acids to discriminate between patients with early nephrosclerosis (chronic kidney disease stage 2–3) and healthy controls. Partial least squares discriminant analysis shows the overall variance between the groups divided into 3 vectors (principal components 1–3). Eighteen amino acids had variable importance in projection scores ≥1.0 (in decreasing order: Hcys, Glu, His, Tyr, Kyn, Car, Cys, Met, Gly, Cth, Lys, Phe, Aaa, Orn, Dap, Ser, DA, Ala), which is considered a significant contribution to discrimination.