Skip to main content
. 2019 Jan 31;9:3191. doi: 10.3389/fimmu.2018.03191

Figure 1.

Figure 1

Involvement of PKCζ in cytokine induced FcαRI activation. (A) Cytokine starved Ba/F3 FcαRI cells were pre-treated for 15 min at 37°C with PKB inhibitor, rapamycin (a p70S6K inhibitor) or PKC inhibitors GF109203X (1 μM) and Ro31-8220 (1 μM). (B) Ba/F3 FcαRI or (D) Ba/F3 FcαRI(p110K227E) were pre-treated for 15 min with indicated concentrations of PKCζ pseudo-substrate. (C) Ba/F3 FcαRI cells overexpressing PKCζ wt or PKC kinase dead (KD) mutant were cytokine starved. (E) Cytokine starved Ba/F3 FcαRI wt or Ba/F3 FcαRI S263A cells were pretreated with 10 μM PKCζ pseudo-substrate (PKCζ PS) for 15 min. All cells were then stimulated with or without IL-3 for 15 min at 37°C. Number of IgA-beads to these cells was measured and IgA binding is expressed as the mean rosette index (number of beads/100 cells) ± SD (n = 3). Values indicated with *differed significantly (p < 0.05) from the control.