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. 2019 Feb 6;9:1513. doi: 10.1038/s41598-018-38064-7

Figure 5.

Figure 5

Inhibition of NADH oxidation by 2B8 is partially restored by CFZ. (a) NADH oxidation by M. smegmatis IMVs was evaluated by monitoring the rate of fluorescence decay at 340ex/460em. Treatment with 2B8 at 78.125–125 µM, 5 mM KCN and 80 µM TRZ potently inhibited NADH oxidation. In contrast, NADH oxidation was minimally inhibited by a lower concentration of 2B8 (62.5 µM) or 125 µM RA13. BDQ at 18 µM had an intermediate inhibitory activity, whereas NADH oxidation was accelerated with 15 µM CCCP. (b,c). Restoration of NADH oxidation by clofazimine (CFZ) was measured in M. smegmatis IMVs treated with 5 mM KCN (b), 80 µM TRZ (b), or 125 µM 2B8 (c). Addition of 42 µM CFZ (dotted lines) restored NADH oxidation in KCN-treated IMVs (b). As expected, CFZ did not restore NADH oxidation in TRZ-treated IMVs (b). In contrast, CFZ also restored NADH oxidation in 2B8-treated IMVs (c), albeit partially in comparison to KCN-treated samples. Experiments were done three separate times and representative data are shown. ***p < 0.001 by ANOVA.