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. 2019 Feb 6;9:1507. doi: 10.1038/s41598-018-37804-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Principal component plot of cell differentiation related gene expression. Differentially expressed gene transcripts related to cell differentiation of cells cultured on TCP, flat, 1 μm, 2 μm and 800 nm silk films (3 samples from each substrates) were used to perform PCA plot of the first three principal components. Profiles group in a similar manner to the hierarchical clustering—the three patterned substrates tend to cluster together.