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. 2019 Feb 6;9:1518. doi: 10.1038/s41598-018-35947-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TFR1 expression is negatively correlated to miR-148a levels in HCC cells. Two HCC cell lines were analyzed for the expression of TFR1 pre-mRNA and mRNA by qPCR. (A) Differences of TFR1 pre-mRNA levels were similar between HepG2 and Huh7 cells (P = 0.7475), while TFR1 mRNA (B) and protein (C) levels were significantly different. (D) miR-148a expression in the HepG2 and Huh7 cell lines. Experiments were performed in triplicates and repeated at least three times. Q-PCR data were normalized to appropriate reference genes: ACTB (A,B) or RNU6 (D). Representative western blots displayed in C are cropped from Fig. S6. Data are represented as mean ± SEM. ns = no statistical significance, *P < 0.05, **P < 0.01, 2-tailed Student’s t test.