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. 2019 Feb 6;9:1518. doi: 10.1038/s41598-018-35947-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of miR-148a decreases TFR1 expression in HCC cells. HepG2 and Huh7 cells were transiently transfected with either a miR-148a mimic, a negative control (NC), specific TFR1 siRNAs (TFR1 siRNA 1 and TFR1 siRNA 2) or a negative control (NC) siRNA. 24 h later, the expression of (A) miR-148a and (B,C) TFR1 mRNA were analyzed by qPCR. TFR1 protein levels were analyzed by western blot analysis following (D) miR-148a overexpression or (E) TFR1 knock-down. Experiments were repeated at least three times. Q-PCR data were normalized to appropriate reference genes: ACTB (B,C) or RNU6 (A). Representative western blots displayed in D and E are cropped from Figs S7 and S8, respectively. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, 2-tailed Student’s t test.