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. 2019 Feb 6;9:1531. doi: 10.1038/s41598-018-38049-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heat Map of Bacterial and Fungal Species. (A) Bacterial heatmap. The top 20 bacterial OTUs for six oral samples processed by eight different extraction methods were used to construct a heatmap. OTUs were classified to species or lowest possible taxonomic level. Heatmap shows that samples cluster by patient (SampleID, 2^nd row), not extraction method (Method, 1^st row). (B) Fungal Heatmap. The top 20 fungal OTUs were used to construct a heatmap for the same samples described in panel A. Fungal OTUs were classified to species or lowest possible taxonomic level. Clustering demonstrated predominant grouping by individual (SampleID, 2^nd row) vs. method of extraction (Method, 1^st row). Legends to the left of the figures indicate color scheme for log transformed OTU abundance, method and sample in descending order.

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