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. 2019 Feb 6;9:1458. doi: 10.1038/s41598-018-37130-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of cultured MLECs by western blot and confocal microscopy. (A,B) RNA-sequencing data mined from published literature¹⁹ shows expression abundance of three EC marker genes, VE-cadherin (also known as CDH5), CD31 (also known as PECAM1), and vWF. Data shown are normalized counts from two different donors of HUVECs. (C) CD31 expression of EC fraction (CD31⁺; ICAM2⁺) and non-EC (CD31⁻; ICAM2⁻) fraction after second bead sorting, n = 3 different mice. (D) VE-cadherin (red) and vWF (green) staining of cultured MLEC by confocal microscopy, n = 4, scale bar = 60 um. (E), DiI-oxLDL uptake (red) by cultured MLECs, n = 5, scale bar = 60 um.