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. 2019 Feb 6;9:1514. doi: 10.1038/s41598-018-37796-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology changes in MCF-7 and MDA-MB-231 cells after eupatorin treatment at 72 hours were recorded using SEM. (A) untreated MCF-7 at 24 hours, (B) untreated MCF-7 at 72 hours, (C) eupatorin-treated MCF-7 cells at 24 hours and (D) eupatorin-treated MCF-7 cells (E) untreated MDA-MB-231 at 24 hours, (F) untreated MDA-MB-231 at 72 hours, (G) eupatorin-treated MDA-MB-231 cells at 24 hours and (H) eupatorin-treated MDA-MB-231 cells at 72 hours. Blue arrow () indicated the membrane collapsed, green arrow () indicated complete loss of cells membrane, and red arrow () indicated shrunken cell and the absence of microvilli while yellow arrow () pointed at intact body of healthy cells. Magnification: x1000; scale bar: 10 μM.

Inline graphic — Morphology changes in MCF-7 and MDA-MB-231 cells after eupatorin treatment at 72 hours were recorded using SEM. (A) untreated MCF-7 at 24 hours, (B) untreated MCF-7 at 72 hours, (C) eupatorin-treated MCF-7 cells at 24 hours and (D) eupatorin-treated MCF-7 cells (E) untreated MDA-MB-231 at 24 hours, (F) untreated MDA-MB-231 at 72 hours, (G) eupatorin-treated MDA-MB-231 cells at 24 hours and (H) eupatorin-treated MDA-MB-231 cells at 72 hours. Blue arrow () indicated the membrane collapsed, green arrow () indicated complete loss of cells membrane, and red arrow () indicated shrunken cell and the absence of microvilli while yellow arrow () pointed at intact body of healthy cells. Magnification: x1000; scale bar: 10 μM.