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. 2019 Feb 6;9:1514. doi: 10.1038/s41598-018-37796-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Eupatorin-induced apoptosis in MCF-7 and MDA-MB-231 cells by AnnexinV-FITC/PI staining assay. The cells were treated with specific concentration of eupatorin for 24, 48 and 72 h. (A) Flow cytometry assay of eupatorin-induced apoptosis cells. (a) untreated for 24 h; (b) untreated for 48 h; (c) untreated for 72 h; (d) treatment with 5 μg/mL eupatorin for 24 h; (e) treatment with 5 μg/mL eupatorin for 48 h; (f) treatment with 5 μg/mL eupatorin for 72 h. (C and D) Columns show mean values of three experiments (±S.D.). (C) eupatorin induced apoptosis of MCF-7 and (D) eupatorin induced apoptosis in MDA-MB-231 cells. (^aStatistical significance (p < 0.05) compared to the untreated for the respective time point, ^bstatistical significance (p < 0.05) compared to the treated group for 24 hours, ^cstatistical significance (p < 0.05) compared to the treated group for 48 hours).