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. 2019 Feb 6;9:1490. doi: 10.1038/s41598-018-37291-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of HDDPC-derived iPSCs. (a) Time-line for isolation of iPSCs from HDDPCs via single, double, or triple transfections. (b) Morphology of the HDDPCs (a) and HDDPC-derived iPSC colonies (b–d). iPSC colonies obtained after single (i), double (ii), or triple transfections (iii) from the HDDPC lines P02, P03, and P04, respectively. The magnified image is shown in the upper right portion of (i). Bar = 500 μm. (c) Immunocytochemistry of HDDPC (P01)-derived iPSC colonies using antibodies for OCT3/4, SOX2, TRA-1–60, and SSEA-4. Cells were stained by DAPI to visualise the location of nuclei. Bar = 500 μm. (d) Immunocytochemistry of embryoid body-derived outgrowth using antibodies for TUJI-1 (ectodermal marker), SMN1 + SMN2 (mesodermal marker), and FOXA2 (endodermal marker). Cells were stained by DAPI to visualise the location of nuclei. Bar = 500 μm.