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. 2019 Feb 6;9:1490. doi: 10.1038/s41598-018-37291-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differentiation induction of the intermediate HDDPCs. (a) Time-line for differentiation induction of the HDDPC line P05 (13 days after the single transfection with Yamanaka’s four reprogramming factors) into neurogenic or osteoblastic lineage. (b) Neuronal cell induction 7 days after incubation in neurogenic medium. Nissl staining demonstrated that Nissl bodies are clearly discernible around the nucleus in the transfected cells (arrowed) in (iii), whereas no such bodies are discernible in the untransfected cells in (i). Bar = 500 μm (i) and (iii), 50 μm (ii) and (iv). (c) Osteoblastic cell induction 15 days after incubation in osteogenic medium. Von Kossa staining in (i) and (iii), and staining with Alizarin red-S in (ii) and (iv). Bar = 500 μm.