Fig. 4.

The partially purified enzyme was checked for the its activity in presence of various substrate. Briefly, the active fractions of DEAE Sephacel and DEAE Sepharose were pooled together. 100 μl of the enzyme the rest of the process was carried out according to standard assay conditions i.e after incubation the reaction mixture was cnterifuged, the supernatant was collected and 100 μl of potassium tetra borate was added to it followed by boiling for 3 min. To this DMAB reagent was added at RT the colour development was measured at 585 nm.