Figure 4.

Introduction of mutations in predicted Can f 6 epitopes. (A) Highly conserved region among Can f 6, Fel d 4, and Equ c 1—but not among other representative lipocalin allergens (indicated by a green bar above the sequences)—was predicted to contain IgE epitope(s) involved in cross-reactivity. Three sites composed of three successive amino acids containing charged residues were substituted with triple alanine, and were designated as rCan f 6-mu-1, mu-2, and mu-3. (B) Schematic representation of the mutation sites (shown in red) in a ribbon diagram (left) and surface model (right) of rCan f 6.