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. 2019 Feb 6;5:62. doi: 10.1038/s41420-018-0134-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Chromosome location of GM26870 and its encoding of three transcripts (splicing variants). GM26870 is located at Chromosome 9: 3,000,282–3,038,313 reverse strand. Shown in the left is the transcript GM26870-201 (1170 bp), shown in the middle is GM26870-202 (1170 bp), and shown in the right is GM26870-203 (1143 bp). Image is copied from the Ensembl website (http://useast.ensembl.org/Mus_musculus/Gene/Splice?db=core;g=ENSMUSG00000097312;r=9:3000282-3038313). b GM26870-201 dsiRNA sequence. The duplex dsiRNA consists of 25 bp in the forward strand and 27 bp in the reverse strand. c Effect of GM26870-201 knockdown on osteoclast formation in mouse BMM. Cell isolation and culture as well as Trap staining were carried out as described in Materials and methods. Shown clearly is that knockdown of GM26870-201 expression blocks osteoclast formation from mouse BMM