Fig. 8. Overexpression of Nampt promotes osteoclast formation.

Bone marrow cells were isolated from male DBA/1J Nampt^+/+ and Nampt^OE mice and differentiated into osteoclasts. TRAP⁺ cells with >3 nuclei were counted as osteoclasts as described in Materials and methods. a Images of TRAP⁺ cells were captured under a light microscope (magnification, 10×). b Quantification of osteoclasts. c A representative image of western blot analysis of Nampt, Nfatc1, and β-Actin protein levels in Nampt^OE BMM. β-Actin was included as a loading control. d Quantification of Nampt and Nfatc1 protein levels. Data were presented as relative expression levels based on the value of Nampt^+/+ wild type (WT) mice as 1. *P < 0.05 vs. Nampt^+/+ mice and ***P < 0.001 vs. Nampt^+/+ WT mice