Skip to main content
. 2019 Feb 6;9:1502. doi: 10.1038/s41598-018-37846-3

Figure 2.

Figure 2

Expression of chemokine receptors CXCR3 and CCR6 in CD4+ T-lymphocytes from TB-HIV co-infected patients prior to and following ART initiation. (A) Frequency of CXCR3+CCR6−, CXCR3−CCR6−, CXCR3+CCR6+ and CXCR3−CCR6+ CD4+ T cells were evaluated in whole blood obtained at week 0 (pre-ART) and at week 6 after ART initiation (in non-IRIS patients, n = 22) or at the time of TB-IRIS event (n = 26). Lines represent median values and interquartile ranges. Data were analyzed using the Mann-Whitney test or Wilcoxon matched-pairs test for paired analyses within each study group. *P < 0.05, **P < 0.01, ***P < 0.001, after adjustment for multiple measurements. (B) Hierarchical cluster analysis of the z-score normalized average frequency values of the CD4+ T cell phenotypes is shown to summarize the overall trends of data variation between the study groups and timepoints. Yellow color represents the highest values whereas blue color indicates the lowest values observed for each cell type. Additional hierarchical cluster analyses of the z-score normalized frequency values of the CD4+ T cell phenotypes per study participant at pre-ART (C) or at week 6 post-ART initiation/time of IRIS event (D) were performed to evaluate whether the overall profile of T cell frequencies could differentiate IRIS from non-IRIS patients. These results were confirmed by principal component analysis (PCA) (C and D, left panels).