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. 2019 Feb 6;9:1502. doi: 10.1038/s41598-018-37846-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Associations between changes in systemic inflammation between before and after ART commencement and frequencies of CD4⁺ T cell subtypes. For the entire study population, change in inflammation over time was estimated by calculating fold-differences of concentrations of each indicated plasma markers as well as for frequencies of the monocyte subsets, from pre-ART to week 2–6 after ART initiation (values at week 2–6 divided by values from pre-ART). Spearman analysis was used to test correlations between changes values of the inflammatory markers and frequencies of the indicated CD4+ T cell subsets in peripheral blood at the time of TB-IRIS event or equivalent timepoint. Colored bars indicate statistically significant correlations (p < 0.05) after adjustment for multiple measurements.