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. 2019 Feb 6;9:1506. doi: 10.1038/s41598-018-37815-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Subcellular localization of selected soybean GmSHMT genes belonging to the four GmSHMT classes. The coding sequences of the indicated GmSHMT genes were fused to the N-terminal end of the eYFP and delivered into onion epidermal cells using biolistic bombardment. YFP fluorescence was localized in the cytoplasm as in the case of Glyma.05g152100 (GmSHMT05c) and Glyma.08G187800 (GmSHMT08c), in the nucleus as in the case of Glyma.06g107800 (GmSHMT06n) and Glyma.08G187800 (GmSHMT08n), or presented a mitochondria-like subcellular localization in case of Glyma.02G217100 (GmSHMT02m) and Glyma.14G184500 (GmSHMT14m), or presented a chloroplastic-like subcellular localization in the case of the Glyma.13G222300 (GmSHMT13ch). No signal was detected in the case of the Glyma.15G089900 (GmSHMT15ch) (Corresponding to a truncated protein and is supposed to be a pseudogene). Bar = 100 µM.