GTTs of saline, single dose and chronic drug treated mice fed on HFD (A,C,E) or chow (B,D,F) diets at week 8, 10 and 12. Mice were fasted for 5 h prior to basal glucose monitoring (as described in Methods section) and subsequently mice were injected I.P. with 20% glucose (w/v), and blood re-analysed at 15, 30, 60 and 90 min post-injection (HFD, n=8 per group; chow, n=4 per group). Data are represented as mean ± S.E.M. and analysed by two-way ANOVA followed by Bonferonni multiple comparison ttests where *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001 when compared with saline treated control mice or ##P≤0.01 when single dose and chronic groups were compared with each other.