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(A–C) Genetic analysis of aortic tissues (n=6 per group) as analysed by qPCR using using SYBR and Light Cycler 480 (Roche). Gene expression of ICAM-1, VCAM-1 and MCP-1 was determined relative to the reference gene ELF1. Data are represented as mean ± S.E.M. and analysed by unpaired two-tailed t tests where *P≤0.05 when compared with saline control.
