Table 1.
Detection of spotted fever group rickettsiae by real-time and conventional PCR for gltA gene.
| Tick species | No. tested (Female/Male/Nymph) | Real-time PCR | Conventional PCR |
|---|---|---|---|
| No. of positive (Female/Male/Nymph) (%) | No. of positive (Female/Male/Nymph) (%) | ||
| A. testudinarium | 85 (3/0/82) | 20 (1/0/19) (23.5) | 16 (1/0/15) (18.8) |
| D. taiwanensis | 12 (7/5/0) | 0 | 0 |
| H. concinna | 7 (2/5/0) | 0 | 0 |
| H. cornigera | 1 (1/0/0) | 0 | 0 |
| H. flava | 128 (59/65/4) | 13 (7/5/1) (10.2) | 11 (6/4/1) (8.6) |
| H. formosensis | 253 (130/122/1) | 7 (2/5/0) (2.8) | 7 (2/5/0) (2.8) |
| H. japonica | 78 (50/25/3) | 4 (2/2/0) (5.1) | 4 (2/2/0) (5.1) |
| H. hystricis | 64 (42/21/1) | 37 (24/13/0) (57.8) | 36 (23/13/0) (56.3) |
| H. kitaokai | 74 (37/36/1) | 3 (0/2/1) (4.1) | 3 (1/1/1) (4.1) |
| H. longicornis | 86 (56/26/4) | 54 (31/22/1) (62.8) | 54 (31/22/1) (62.8) |
| H. megaspinosa | 201 (106/92/3) | 35 (21/14/0) (17.4) | 27 (16/11/0) (13.4) |
| H. yeni | 1 (1/0/0) | 0 | 0 |
| I. monospinosus | 58 (38/20/0) | 34 (20/14/0) (58.6) | 34 (20/14/0) (58.6) |
| I. nipponensis | 5 (0/5/0) | 4 (0/4/0) (80) | 4 (0/4/0) (80) |
| I. ovatus | 652 (339/313/0) | 7 (4/3/0) (1.1) | 7 (4/3/0) (1.1) |
| I. pavlovskyi | 33 (16/17/0) | 0 | 0 |
| I. persulcatus | 446 (220/222/4) | 155 (87/68/0) (34.8) | 150 (82/68/0) (33.6) |
| I. tanuki | 2 (1/1/0) | 0 | 0 |
| I. turdus | 3 (3/0/0) | 0 | 0 |
| Total | 2,189 (1,111/975/103) | 373 (199/152/22) (17.0) | 352 (187/147/18) (16.1) |