Table 3.
Results of PCR amplification for the ompA, ompB, htrA, sca4 and 16S rRNA genes.
| gltA genotype | Tick species | No. tested | Mean rickettsial burden (copies/µl)* | PCR amplification | ||||
|---|---|---|---|---|---|---|---|---|
| ompA | ompB | htrA | sac4 | 16S rRNA | ||||
| G1 | H. megaspinosa | 2 | 7.9E + 3 | − | + | + | + | + |
| G2 | H. hystricis | 2 | 1.1E + 4 | − | + | + | + | + |
| G3 | I. persulcatus | 4 | 8.7E + 3 | + | − | + | − | + |
| G4 | I. persulcatus | 3 | 8.4E + 3 | − | + | + | − | + |
| G5 | I. persulcatus | 6 | 1.3E + 3 | − | − | + | − | − |
| G5 | I. monospinosus | 6 | 2.3E + 2 | − | + | + | + | + |
| G6 | H. longicornis | 2 | 2.4E + 3 | + | + | + | + | + |
| G7 | H. formosensis | 1 | 1.0E + 4 | + | + | + | + | + |
| G8 | A. testudinarium | 3 | 2.1E + 4 | + | + | + | − | + |
| G9 | H. kitaokai | 2 | 1.6E + 4 | − | + | + | − | + |
| G10 | I. nipponensis | 2 | 3.0E + 3 | + | + | + | − | + |
| G11 | H. japonica | 3 | 2.6E + 3 | − | + | + | + | + |
| G11 | H. flava | 7 | 1.8E + 3 | − | − | + | − | − |
| G12 | H. flava | 3 | 1.2E + 3 | − | − | + | − | − |
| G13 | I. ovatus | 5 | 2.5E + 3 | − | + | + | − | + |
| G14 | H. formosensis | 3 | 3.6E + 3 | − | − | + | − | − |
| G15 | H. formosensis | 3 | 1.3E + 3 | − | − | + | − | − |
+, Amplified; −, Not amplified. *The mean copy number of rickettsial gltA gene in the template DNA was calculated by gltA real-time PCR.