Table 4.
Primers uesd in the present study.
| Primer | Sequence (5′-3′) | Target gene | Annealing temperature (°C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
| CS-F | TCGCAAATGTTCACGGTACTTT | citrate synthase gene (gltA) | 60 | 74 | Steno et al.43 |
| CS-R | TCGTGCATTTCTTTCCATTGTG | ||||
| CS-P | TGCAATAGCAAGAACCGTAGGCTGGATG | ||||
| gltA_Fc | CGAACTTACCGCTATTAGAATG | citrate synthase gene (gltA) | 55 | 580 | Gaowa et al.16 |
| gltA_Rc | CTTTAAGAGCGATAGCTTCAAG | ||||
| Rr.190.70p | ATGGCGAATATTTCTCCAAAA | outer membrane A gene (ompA) | 48 | 542 | Regnery et al.44 |
| Rr.190.602n | AGTGCAGCATTCGCTCCCCCT | ||||
| 120_2788 | AAACAATAATCAAGGTACTGT | outer membrane B gene (ompB) | 48 | 816 | Roux and Raoult45 |
| 120_3599 | TACTTCCGGTTACAGCAAAGT | ||||
| 17k_5 | GCTTTACAAAATTCTAAAAACCATATA | 17-kDa common antigen gene (htrA) | 52 | 550 | Labruna et al.47 |
| 17k_3 | TGTCTATCAATTCACAACTTGCC | ||||
| Rick_16S_F3 | ATCAGTACGGAATAACTTTTA | 16S ribosomal RNA gene (16S rRNA) | 52 | 1328 | Anstead et al.48 |
| Rick_16S_F4 | TGCCTCTTGCGTTAGCTCAC | ||||
| rrs2_seq_1 | AGGCCTTCATCACTCACTCG* | This study | |||
| rrs2_seq_2 | CTACACGCGTGCTACAATGG* | ||||
| D1f | ATGAGTAAAGACGGTAACCT | surface cell antigen-4 (sca4) | 50 | 928 | Sekeyova et al.46 |
| D928r | AAGCTATTGCGTCATCTCCG | ||||
| sca4_seq_1 | GCCGGCTATTTCTATTGATTC* | This study | |||
| sca4_seq_2 | TGCAAGCGATCTTAGAGCAA* | This study |
*The primers were used for sequencing.