FIG 4.

Differentially regulated metabolic fluxes and metabolites in gluconeogenesis (A), pentose phosphate pathway (B), TCA cycle (C), arginine biosynthesis pathways (D), and respiratory chain (E) under 2-mg/liter colistin treatment for 1 h. The specific flux values under control and colistin treatment are denoted in the format flux_control/flux_colistin. Significantly altered metabolites from metabolomics data are highlighted in purple with fold change to the side. The metabolite abbreviations are as follows: G6P, glucose 6-phosphate; F6P, fructose 6-phosphate; FBP, fructose 1,6-biphosphate; DHAP, dihydroxyacetone phosphate; G3P, glyceraldehyde 3-phosphate; 1,3-DPG, 1,3-bisphosphoglycerate; 3PG, 3-phosphoglycerate; 2PG, 2-phosphoglycerate; PEP, phosphoenolpyruvate; PYR, pyruvate; MAL, (S)-malate; OAA, oxaloacetate; Ru5P, ribulose 5-phosphate; R5P, ribose 5-phosphate; Xu5P, xylulose 5-phosphate; S7P, sedoheptulose 7-phosphate; PRPP, phosphoribosyl pyrophosphate; AcCoA, acetyl-CoA; CIT, citrate; ACON, cis-aconitate; ICIT, isocitrate; α-KG, α-ketoglutarate; SUCC-CoA, succinyl-CoA; SUCC, succinate; FUM, fumarate; ARG, l-arginine; GLU, l-glutamate; NAcGLU, N-acetyl-glutamate; NAcGLUP, N-acetyl-γ-glutamyl-phosphate; NAcGLU5SAD, N-acetyl-l-glutamate-5-semialdehyde; NAcORN, N-acetyl-ornithine; ORN, l-ornithine; CP, carbamoyl phosphate; HCO₃, bicarbonate; CITR, l-citrulline; ARGSUCC, argininosuccinate; UQL, ubiquinol-8; UQN, ubiquinone-8; IM, inner membrane.