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. 2019 Jan 9;41(3):1707–1717. doi: 10.3892/or.2019.6961

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Copyright: © Zheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — AQP4 promotes oxygen glucose deprivation (OGD)/RX-induced astrocyte cell injury. (A and B) Cell viability assay of OGD/RX-treated primary cultured astrocytes transfected with miR-29 mimic, or negative controls (NC), following propidium iodide (PI)/calcein staining. ***P<0.001 vs. NC; ^###P<0.001 vs. OGD/RX+NC (n=3 for each experiment). (C) Expression of LDH determined by ELISA. ***P<0.001 vs. NC; ^###P<0.001 vs. OGD/RX+NC (n=3). (D and E) Flow cytometric analysis of apoptotic cells among OGD/RX-induced astrocytes transfected with miR-29a mimic or NC. ***P<0.001 vs. NC; ^###P<0.001 vs. OGD/RX+NC (n=3). (F and G) Cell heath assay of OGD/RX-treated primary cultured astrocytes transfected with miR-29 inhibitor or NC followed by PI/calcein staining. **P<0.01, ***P<0.001 vs. NC; ^##P<0.01, ^###P<0.001 vs. OGD/RX+inhibitor NC (n=3). (H) Expression of LDH detected by ELISA. ***P<0.001 vs. NC; ^###P<0.001 vs. OGD/RX+inhibitor NC (n=3 for each experiment). (I and J) Rate of apoptosis determined by flow cytometry in OGD/RX-induced astrocytes transfected with miR-29a inhibitor or NC. ***P<0.001 vs. NC; ^#P<0.05 vs. OGD/RX+inhibitor NC (n=3 for each experiment). (K and L) Western blotting of the expression of Bax and Bcl-2 in OGD/RX-induced astrocytes transfected with miR-29a mimic, inhibitor, or NC. OGD/RX, oxygen glucose deprivation-reoxygenation; AQP4, aquaporin 4; PI, propidium iodide.

Figure 2. — AQP4 promotes oxygen glucose deprivation (OGD)/RX-induced astrocyte cell injury. (A and B) Cell viability assay of OGD/RX-treated primary cultured astrocytes transfected with miR-29 mimic, or negative controls (NC), following propidium iodide (PI)/calcein staining. ***P<0.001 vs. NC; ^###P<0.001 vs. OGD/RX+NC (n=3 for each experiment). (C) Expression of LDH determined by ELISA. ***P<0.001 vs. NC; ^###P<0.001 vs. OGD/RX+NC (n=3). (D and E) Flow cytometric analysis of apoptotic cells among OGD/RX-induced astrocytes transfected with miR-29a mimic or NC. ***P<0.001 vs. NC; ^###P<0.001 vs. OGD/RX+NC (n=3). (F and G) Cell heath assay of OGD/RX-treated primary cultured astrocytes transfected with miR-29 inhibitor or NC followed by PI/calcein staining. **P<0.01, ***P<0.001 vs. NC; ^##P<0.01, ^###P<0.001 vs. OGD/RX+inhibitor NC (n=3). (H) Expression of LDH detected by ELISA. ***P<0.001 vs. NC; ^###P<0.001 vs. OGD/RX+inhibitor NC (n=3 for each experiment). (I and J) Rate of apoptosis determined by flow cytometry in OGD/RX-induced astrocytes transfected with miR-29a inhibitor or NC. ***P<0.001 vs. NC; ^#P<0.05 vs. OGD/RX+inhibitor NC (n=3 for each experiment). (K and L) Western blotting of the expression of Bax and Bcl-2 in OGD/RX-induced astrocytes transfected with miR-29a mimic, inhibitor, or NC. OGD/RX, oxygen glucose deprivation-reoxygenation; AQP4, aquaporin 4; PI, propidium iodide.