Figure 2. Effect of PD98059, ERK inhibitor on cell viability, NF-ĸB activation, and metastasis related proteins and ERK phosphorylation in SKHep1cells. Cells were treated with 0-20 μM PD98059 for 48 h. A) Change of cell viability was analyzed with the MTT assay. B) NF-ĸB reportergene assay was used to verify NF-ĸB activation. C) Protein expression of MMP-2, MMP-9, uPA, phosphor-ERK (pERK) was assayed by westernblot assays. *p<0.05 and **p<0.01 compared to control (0.1 % DMSO treatment).
