Figure 4. Stimulating effect of trans-cinnamaldehyde (CMA) and methylcinnamate (MC) on cyclo-oxygenase-2 (Cox2), nitric oxide synthase 2(Nos2) and heme oxygenase 1 (Ho1) gene expression in RAW264.7 cells.The cells were incubated for 3.5 h with each compound at aconcentration of 0.1-10 mM, and then their total RNAs were prepared.Each cDNA was synthesized, and the expression level of each mRNA wasdetermined by real-time polymerase chain reaction and standardizedagainst the expression of 18s rRNA. The results are presented asmeans±standard error (SE) of three independent experiments, SE<15%.*Significantly different at p<0.01 vs. control group .